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nikon microscopy  (Nikon)


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    Nikon nikon microscopy
    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence <t>microscopy</t> of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software

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    Microscopy:

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    Article Snippet: .. The random fields of each well were captured with Nikon microscopy. .. Total RNA was extracted by TRizol (YEASEN, China) from LX-2 cells, transfected with POSTN-overexpressed vector, and treated with H128 CM or H69 CM for 48 h and RNase-free DNase I to remove genomic DNA contamination.

    Article Title: AMPK phosphorylation of K Ca 2.3 alleviates angiotensin II-induced endothelial dysfunction.
    Article Snippet: The endothelial small-conductance calcium-activated potassium channels (KCa2.3) are indispensable for endothelium-dependent hyperpolarization (EDH) response, mainly in resistance arteries.. We recently demonstrated in diet-induced obese mice that adenosine monophosphate-activated protein kinase (AMPK) upregulates endothelial KCa2.3 expression and improves endothelial function.. However, the molecular mechanism of regulation of KCa2.3 by AMPK remains less explored.

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    Article Snippet: .. During the image collection, the x-y drift was corrected using 1 μm multicolor S6 microspheres (Invitrogen) and the z-drift was eliminated using Perfect Focus System provided by Nikon microscopy. ..

    Article Title: Activation of atrial natriuretic peptide receptor A depresses hypothalamic PVN CRFergic neuronal excitability by inhibiting IH in vitro in mice.
    Article Snippet: Atrial natriuretic peptide (ANP) and its receptor are abundantly distributed in hypothalamic paraventricular nucleus (PVN), and contribute to regulation of corticotropin releasing factor (CRF) release in the brain.. However, the mechanism by which ANP regulates the activity of PVN CRFergic neurons remains unclear.. Here, we investigated the mechanism by which ANP modulates the neuronal activity related to PVN CRF-mRNA expression in vitro in mice using whole-cell patch-clamp recording, single-cell RT-PCR, immunofluorescence, and pharmacological methods.

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7
    Article Snippet: .. Three-dimensional structures were reconstructed using NIS Elements imaging software for Nikon microscopy (Nikon, Japan). ..

    Article Title: Chronic low-dose cadmium exposure induces neurodevelopmental impairment in hESC-derived retinal organoids.
    Article Snippet: .. After cadmium exposure, retinal organoids were photographed using Nikon microscopy (ECLIPSE Ti2, Nikon Corporation, Tokyo, Japan) to observe the morphology and growth. .. ImageJ software (Version 1.52a, NIH, Bethesda, MD, USA) was used to identify the NR or non-NR structure, and measure the thickness and area of NRs, as well as the entire area of retinal organoids.

    Article Title: N6-methyladenosine demethylase FTO enhances chemo-resistance in colorectal cancer through SIVA1-mediated apoptosis
    Article Snippet: Tumor growth curve (C) and tumor weight (D) are shown, n = 6. ( H&E and immunohistochemical images of Ki67, Bcl-2, and c-Caspase-3 in CRC-PDX histochemical images by IPP analysis. (J and K) Up-regulation of SIVA1 was highly ass mation: In all relevant panels, ns, not significant, *p < 0.05; **p < 0.01; ***p < 0.001; ** For EdU (5-Ethynyl-20-deoxyuridine) assay, logarithmic growth stage cells with treated or not were seeded in a 12-well plate with corresponding concentration of EdU reagent for 2 h. Cells were washed with PBS for 5 min twice, before incubating with 4% paraformaldehyde for 30 min. After washing with PBS for 5 min twice, samples were permeated with 0.3% Triton X-100 in PBS, and dyed with a reaction solution (C0075S, Beyotime, China). .. Images collected with 20 and 40 visions in Nikon microscopy. .. For cell apoptosis assays, cells were performed using Annexin V-PI Apoptosis Detection Kit I (WLA001a, Wanleibio, China) according to the manufacturer’s instruction, and followed by flow cytometry analysis (Beckman, USA).

    Article Title: N6-methyladenosine demethylase FTO enhances chemo-resistance in colorectal cancer through SIVA1-mediated apoptosis
    Article Snippet: .. Lung tissues were harvested and fixed with 4% PFA for paraffinembedded section and lung metastases were detected with Nikon microscopy. ..

    Fluorescence:

    Article Title: AMPK phosphorylation of K Ca 2.3 alleviates angiotensin II-induced endothelial dysfunction.
    Article Snippet: The endothelial small-conductance calcium-activated potassium channels (KCa2.3) are indispensable for endothelium-dependent hyperpolarization (EDH) response, mainly in resistance arteries.. We recently demonstrated in diet-induced obese mice that adenosine monophosphate-activated protein kinase (AMPK) upregulates endothelial KCa2.3 expression and improves endothelial function.. However, the molecular mechanism of regulation of KCa2.3 by AMPK remains less explored.

    Patch Clamp:

    Article Title: Activation of atrial natriuretic peptide receptor A depresses hypothalamic PVN CRFergic neuronal excitability by inhibiting IH in vitro in mice.
    Article Snippet: Atrial natriuretic peptide (ANP) and its receptor are abundantly distributed in hypothalamic paraventricular nucleus (PVN), and contribute to regulation of corticotropin releasing factor (CRF) release in the brain.. However, the mechanism by which ANP regulates the activity of PVN CRFergic neurons remains unclear.. Here, we investigated the mechanism by which ANP modulates the neuronal activity related to PVN CRF-mRNA expression in vitro in mice using whole-cell patch-clamp recording, single-cell RT-PCR, immunofluorescence, and pharmacological methods.

    Imaging:

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7
    Article Snippet: .. Three-dimensional structures were reconstructed using NIS Elements imaging software for Nikon microscopy (Nikon, Japan). ..

    Software:

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7
    Article Snippet: .. Three-dimensional structures were reconstructed using NIS Elements imaging software for Nikon microscopy (Nikon, Japan). ..



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    Image Search Results


    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Three-dimensional structures were reconstructed using NIS Elements imaging software for Nikon microscopy (Nikon, Japan).

    Techniques: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software